Lecture on Anatomy of the Retina – Structure, Layers & Functions

Anatomy of the Retina

The retina is a thin, transparent, delicate layer of neural tissue lining the inner surface of the posterior segment of the eye. It is the most highly developed tissue of the eye. It appears purple red due to the visual purple of rods and the underlying vascular choroid. It plays a crucial role in vision by converting light into neural signals, which are then transmitted to the brain for image processing. Understanding the anatomy of the retina is fundamental for comprehending how vision works and for diagnosing and treating various ocular diseases.

Anatomy of the Retina

Gross Anatomy

Location and Structure

Located between the choroid (outside) and the vitreous body (inside).

Extends from the optic disc (where the optic nerve exits the eye) to the ora serrata (the anterior margin).

Approximately 0.1–0.5 mm thick, thickest near the optic disc and thinnest at the ora serrata.

The posterior pole of retina is best examined by slit lamp, indirect ophthalmoscopy using +78D and +90D lens, and direct ophthalmoscopy.

Regions of the Retina

Optic Disc

Pink colored, well-defined circular area of 1.5 mm diameter.

All retinal layers terminate here except the nerve fiber layer, which passes through the lamina cribrosa to form the optic nerve.

A depression called the physiological cup is present.

Central retinal artery and vein enter through the center of this cup.

Lacks photoreceptors.

Macula Lutea

Also called the yellow spot.

Slightly deeper red than surrounding fundus, located temporal to the optic disc.

Central area of the retina responsible for high-acuity vision.

About 5.5 mm in diameter.

Contains a central depression known as the fovea centralis.


Fovea Centralis

Small depression in the macula.

Contains only cone photoreceptors → responsible for sharp central vision.

About 1.5 mm in diameter, most sensitive part of retina.

Center contains a shiny pit called foveola (~0.35 mm).

Located ~2 disc diameters temporal to optic disc and 1 mm below horizontal meridian.

No rods present.

Cones are tightly packed; other retinal layers very thin.

Only the internal limiting membrane is present.

The area (~0.8 mm diameter) around the foveola without retinal capillaries is called the foveal avascular zone.

Surroundings: parafoveal and perifoveal regions.

Peripheral Retina

Outside the macula.

Contains more rods → peripheral & night vision.

Best examined with indirect ophthalmoscopy and Goldmann’s three-mirror contact lens.

Ora Serrata

Serrated anterior margin where retina ends.

Firmly attached to vitreous and choroid.

Pars plana extends anteriorly from ora serrata.

Microscopic Anatomy

Layers of the Retina (10 layers, outer → inner)

1. Retinal Pigment Epithelium (RPE)

Outermost layer, single layer of pigmented cells.

Absorbs stray light, supports photoreceptors, visual cycle.

Firmly attached to basal lamina of choroid.


2. Photoreceptor Layer (rods & cones)

End-organs of vision (120 million rods, 6.5 million cones).

Rods: contain rhodopsin (visual purple), for peripheral & scotopic vision (low light).

Cones: contain photopsins, for central & color (photopic) vision.


3. External Limiting Membrane

Formed by junctions between photoreceptors & Müller cells.


4. Outer Nuclear Layer

Cell bodies (nuclei) of rods & cones.


5. Outer Plexiform Layer

Synapses between photoreceptors, bipolar cells, and horizontal cells.


6. Inner Nuclear Layer

Contains cell bodies of bipolar, horizontal, amacrine, and Müller cells.

Bipolar cells = first-order neurons.


7. Inner Plexiform Layer

Synapses between bipolar, amacrine, and ganglion cells.


8. Ganglion Cell Layer

Cell bodies of ganglion cells.

Ganglion cells = second-order neurons of visual pathway.


9. Nerve Fiber Layer

Axons of ganglion cells → converge to form optic nerve.


10. Internal Limiting Membrane

Innermost layer.

Formed by footplates of Müller cells.

Types of Cells in the Retina

Photoreceptors

Rods → low light, night vision.

Cones → color vision, high acuity.

Bipolar Cells

Relay from photoreceptors → ganglion cells.

Ganglion Cells

Axons form optic nerve → transmit visual signals to brain.

Horizontal Cells

Integrate/regulate input from multiple photoreceptors.

Amacrine Cells

Modulate transmission between bipolar & ganglion cells.

Müller Glial Cells

Provide structural & metabolic support.

Blood Supply

Central Retinal Artery → inner 2/3 of retina.

Choroidal Circulation → outer 1/3 including photoreceptors.

Clinical Relevance

Retinal Detachment → separation from RPE, vision loss.

Macular Degeneration → affects central vision.

Diabetic Retinopathy → vascular damage due to diabetes.

Retinitis Pigmentosa → genetic, progressive photoreceptor loss.

Retina of eye


Summary

The retina is a complex multilayered structure crucial for vision. Specialized cells and layers convert light into neural signals, processed by the brain to form visual images. Knowledge of retinal anatomy is essential for diagnosing and treating ocular diseases.
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